动脉粥样硬化性心血管疾病患者血清miR-103b检测的临床价值
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国家自然科学基金(81272360) ;东部战区总医院院内课题( YYQN2021077,49156)


Clinical value of serum miR-103b levels in patients with atherosclerotic cardiovascular disease
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    摘要:

    摘要:目的探讨动脉 粥样硬化性心血管疾病( atherosclerosis cardiovascular disease, ASCVD)患者血清miR-103b的表达水平变化及其临床应用价值。方法选取2021 年1月至2022年11月在东部战区总医院心血管内科确诊的ASCVD患者以及同 期体检的健康人对照血清标本各80例。采用实时荧光定量PCR( qRT-PCR)检测各组血清miR-103b的表达水平;运用ROC .曲线、Spearman相关性分析血清miR-103b 对ASCVD 的预测及评估价值。结果qRT-PCR 结果显示,与健康人对照组[0.000 2(0.000 08 ,0.000 4)]相比, ASCVD患者血清miR- 103b的表达水平[0.000 9(0.000 5,0.003 3)]显著升高,差异有统计学意义(U=1 069, P<0.001)。Spearman 相关分析显示,血清miR-103b与心肌肌钙蛋白I(r=0.322 ,P<0.05)、心肌肌钙蛋白T(r= 0.298 ,P<0.05)、肌酸激酶MB同工酶(r=0.393,P<0.05)、总胆固醇(r=0.290, P<0.001).低密度脂蛋白胆固醇(r=0.268 ,P<0.001)均呈正相关,而与高密度脂蛋白胆固醇(r=-0.313,P<0.001)呈负相关。ROC曲线分析结果显示,血清miR-103b水平区分ASCVD患者及健康人对照者的ROC曲线下面积( AUCROC)为0.854( 95%CI:0.791 ~0.917),敏感性为73.26% ,特异性为83.92%。结论ASCVD 患者血清miR-103b的表达水平上调,可作为ASCVD潜在的辅助诊断生物学标志物。

    Abstract:

    Abstract : Objective To investigate the expression and clinical value of miR- 103b in serum of the patients with atherosclerosis cardio-vascular disease ( ASCVD). Methods The serum samples were collected from 80 ASCVD patients who were diagnosed in the Department of Cardiology, General Hospital of Eastern Theater Command from January 2021 to November 2022, and 80 serum samples of healthy controls were simultaneously collcted. Quantitative real-time polymerase chain reaction ( qRT-PCR) was used to detect the expression level of miR- 103b in serum. ROC curve and Spearman corelation analysis were used to evaluate the predictive value of serum miR-103b for ASCVD. Results Compared to the control group [0.0002 (0.000 08, 0.000 3)], the expression level of miR-103b in the serum of ASCVD group [0.001 7(0.000 8, 0.003 3) ] was significantly up-regulated (U= 1 069, P<0.001 ). Correlation analysis showed that miR- 103b was positively correlated with cardiac troponinI (eTnI) (r=0.322, P<0.05), cTnT (r=0.298, P<0.05), creatine kinase-MB (CK-MB) (r= 0.393, P<0.05), cholesterol (r=0.290, P<0.001 ), low density lipoprotein cholesterol (r=0.268,P<0.001),and was negatively correlated with high density lipoprotein cholesterol (r=-0.313, P<0.001). The ROC curve analysis showed that the area under the ROC curve ( AUCROC ) of serum miR-103b levels distinguishing ASCVD patients and healthy controls was 0.854 (95% CI:0.791-0.917) with sensitivity of 73.26% and speificity of 83.92%. Conclusion The expression of miR-103b in .serum of the patients with ASCVD is significantly up-regulated compared with healthy control. The level of miR- 103b could be used as a potential molecular biomarker for auxiliary diagnosis of ASCVD.

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母迷迷,吴嘉,王苏梦,张乐乐,张钰,薛丽君,丁小宇,汪俊军.动脉粥样硬化性心血管疾病患者血清miR-103b检测的临床价值[J].临床检验杂志,2024,42(003):166-171

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  • 收稿日期:2023-07-18
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  • 在线发布日期: 2024-05-09
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