国家自然科学基金(81871720)
目的 利用生信分析筛选胃癌中的差异表达基因(DEGs),构建胃癌竞争性内源 RNA(ceRNA)调控网络,预测胃癌诊治 的潜在靶点。方法 对 TCGA 数据库和 GEO 数据库中获得的测序数据进行差异基因分析,挖掘胃癌中差异表达的 LncRNAs、 mRNAs 和 miRNAs,并构建 ceRNA 网络。利用在线 DAVID 数据库对差异基因进行 GO 分析和 KEGG 分析。利用荧光定量 PCR对部分差异基因进行临床样本验证。结果 共筛选出 95 个差异基因,可能参与受体配体激活、信号受体激活、丝氨酸型 肽酶激活、丝氨酸水解酶激活、化学排斥物激活以及跨膜受体蛋白络氨酸激酶激活等生物学过程,影响癌症中的转录调节失 调、癌症中的 miRNAs 等信号通路。结论 成功构建了参与胃癌发生、发展的 LncRNAmiRNAmRNA 调控网络,并对部分基因 进行临床标本验证,为胃癌诊断和治疗的分子生物学研究提供新的依据。
Objective To screen the differential expressed genes (DEGs)in gastric cancer (GC)by bioinformatic analysis,and con struct the regulatory network of competitive endogenous RNA (ceRNA)to predict the potential targets for diagnosis and treatment of GC. Methods The sequencing data of the DEGs obtained from TCGA and GEO database were analyzed to explore the differentially ex pressed lncRNAs,mRNAs and miRNAs in GC and construct ceRNA network. GO and KEGG analysis for DEGs were performed using online DAVID database. Fluorescence quantitative PCR was used to verify some DEGs in clinical samples. Results A total of 95 dif ferential genes were screened,which may be involved in the biological processes of activation of receptorligand,signalreceptor,serine type peptidase,serine hydrolase,chemorepellent and tyrosine kinase of transmembrane receptor protein. The transcriptional misregula tion,microRNAs and other signaling pathways in cancer were affected. Conclusion The lncRNAmiRNAmRNA regulatory network involved in development and progression of GC was successfully constructed,and some of the genes were verified by clinical speci mens,providing new theoretical evidences for molecular biological research on diagnosis and treatment of GC.
冯伟,宗炜,鞠少卿.基于 TCGA 和 GEO 数据库构建胃癌 ceRNA 调控网络[J].临床检验杂志,2021,39(9):711-715