目的 探讨微小 RNA155(miR155)、长链非编码 RNA MIR155 宿主基因(LncRNA MIR155HG)在肺结核患者外周血单 个核细胞(PBMC)中的表达水平,并分析其临床筛查价值。方法 选取阿坝藏族羌族自治州人民医院诊治的肺结核患者 130 例作为肺结核组,其中活动性肺结核(ATB)患者 56 例(ATB 组)、潜伏结核感染(LTBI)患者 74 例(LTBI 组),并以体检健康者 134 例作为健康人对照组。采用实时荧光定量 PCR(qRTPCR)检测各组 PBMC 中 LncRNA MIR155HG、miR155 的表达水平; 分析 ATB 患者 PBMC 中LncRNA MIR155HG 的表达水平与 miR155 的相关性,并评估二者对 ATB 患者的临床筛查价值。结果 肺结核组患者 PBMC 中 LncRNA MIR155HG、miR155 的表达水平[(1.78±0.57)、(1.65±0.54)]均显著高于健康人对照组 [(1.02±0. 32)、(1. 01 ± 0.33)],差异有统计学意义(t 分别为 13. 410、11. 658,P 均 < 0. 05);ATB 组患者 PBMC 中 LncRNA MIR155HG、miR155 的表达水平[(2.02±0.68)、(1.90±0.63)]亦显著高于 LTBI 组[(1.55±0.53)、(1.41±0.46)],差异有统计学 意义(t分别为 4.430、5.127,P均<0.05);未治愈组 ATB 患者 PBMC 中 LncRNA MIR155HG、miR155 的表达水平[(2.34±0.78)、 (2.15±0.72)]显著高于治愈组[(1.83±0.61)、(1.75±0.58)],差异有统计学意义(t 分别为 2.725、2.280,P 均<0.05)。ATB、 LTBI患者 PBMC 中 LncRNA MIR155HG 的表达均与 miR155 呈正相关(r 分别为 0. 461、0. 397,P 均 < 0. 05);LncRNA MIR155HG、miR155 筛查 ATB 的 ROC 曲线下面积(AUCROC)分别为 0. 824(95% CI:0. 749 ~ 0. 900)、0. 843(95% CI:0. 772 ~ 0.914),当 cutoff 值分别为 1.81、1.72 时,其敏感性分别为 71.4%、73.2%,特异性分别为 87.8%、86.5%;二者联合检测筛查 ATB 的 AUCROC为 0.917(95%CI:0.871~ 0.964),当 cutoff 值为 0.46 时,其敏感性、特异性分别为 89.3%、82.4%。结论 肺结核患者 PBMC中 LncRNA MIR155HG、miR155 的表达水平较高,且在 ATB 患者中的表达水平显著高于 LTBI 患者,二者联合检测有助 于临床筛查 ATB,评估患者病情。
Objective To investigate the expression levels of microRNA155 (miR155)and long noncoding RNA MIR155 host gene (LncRNA MIR155HG)in peripheral blood mononuclear cells (PBMC)of the patients with pulmonary tuberculosis and their diagnostic value. Methods A total of 130 patients with pulmonary tuberculosis,including 56 patients with active pulmonary tuberculosis (ATB) and 74 patients with latent tuberculosis infection (LTBI),and 134 healthy controls were enrolled in our study. The expression levels of LncRNA MIR155HG and miR155 in PBMC were detected by realtime fluorescence quantitative PCR (qRTPCR). The relationship between the expression levels of LncRNA MIR155HG and miR155 in PBMC of ATB patients was analyzed,and their diagnostic values were evaluated. Results The expression levels of LncRNA MIR155HG and miR155 in PBMC of the patients with pulmonary tubercu losis (1.78±0.57 and 1.65±0.54)were significantly higher than those of healthy controls (1.02±0.32 and 1.01±0.33;t = 13.410 and 11.658,P<0.05). The expression levels of LncRNA MIR155HG and miR155 in PBMC of the ATB patients (2.02±0.68 and 1.90± 0.63)were significantly higher than those of the LTBI patients (1.55±0.53 and 1.41±0.46,t = 4.430 and 5.127,P<0.05). The ex pression levels of LncRNA MIR155HG and miR155 in PBMC of the ATB patients without remission (2.34±0.78 and 2.15±0.72)were significantly higher than those cured (1.83±0.61 and 1.75±0.58,t = 2.725 and 2.280,P<0.05). The expression levels of LncRNA MIR155HG in PBMC of ATB and LTBI patients were positively correlated with those of miR155 (r = 0.461 and 0.397,P<0.05). The areas under the receiver operating characteristics (ROC)curve (AUCROC)of LncRNA MIR155HG and miR155 for the screening of ATB were 0.824 (95% CI:0. 7490. 900)and 0. 843 (95% CI:0. 7720. 914),respectively. When the cutoff values of LncRNA MIR155HG and miR155 were 1.81 and 1.72,respectively,the sensitivity were 71.4% and 73.2%,and specificity were 87.8% and 86.5%,respectively. The AUCROC of the combined detection of LncRNA MIR155HG and miR155 for the screening of ATB was 0.917 (95%CI:0.8710.964). When the cutoff value was 0.46,the sensitivity and specificity were 89.3% and 82.4%,respectively. Conclusion The expression levels of LncRNA MIR155HG and miR155 in PBMC of patients with pulmonary tuberculosis are higher,and the expression levels in ATB patients are significantly higher than that in LTBI patients. The combined detection of LncRNA MIR155HG and miR155 is helpful for the clinical screening of ATB and evaluation of the patient's condition.
李君莉,赵慧姝.外周血 LncRNA MIR155HG、miR155 检测在肺结核筛 查中的临床价值[J].临床检验杂志,2021,39(9):675-678