用MALDI-TOF MS技术快速鉴定临床分离棒状杆菌属细菌
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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid identification of Corynebacterium species of clinical isolates
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    摘要:目的:评价基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF MS)快速鉴定临床分离的非白喉棒状杆菌的价值。 方法:收集本院58株非白喉棒状杆菌,用MALDI-TOF MS和16S rRNA基因测序两种方法进行鉴定和比较。用MALDI-Biotyper软件构建不同棒状杆菌MALDI-TOF MS蛋白系统发育树。 结果:58株非白喉棒状杆菌中,54株MALDI-TOF MS与16S rRNA基因测序鉴定结果一致,包括34株纹带棒状杆菌(Corynebacterium straitum)、11株杰氏棒状杆菌(C.jeikeium)、3株C.resistens、2株解葡萄糖苷棒状杆菌(C.glucuronolyticum)、2株黏金色棒状杆菌(C.aurimucosum)和2株无枝菌酸棒状杆菌(C.amycolatum)。4株16S rRNA基因测序无法鉴定到种的菌株中,MALDI-TOF MS鉴定为2株产黏棒状杆菌(C.mucifaciens)、1株C.singulare和1株假白喉棒状杆菌(C.commune)。 结论:MALDI-TOF MS可将棒状杆菌属细菌快速、准确地鉴定到种。

    Abstract:

    Abstract: Objective:To evaluate the application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid identification ofCorynebacterium spp. Methods:Fifty-eight clinical isolates of Corynebacterium spp. were collected. Species identification was performed by both MALDI-TOF MS and 16S rRNA gene sequencing. MALDI-TOF MS protein phylogenetic tree of different Corynebacterium species was constructed by MALDI-Biotyper software. Results:The results of identification for 54 isolates by 16S rRNA gene sequencing were coincident with those by MALDI-TOF MS, including 34 of C.straitum strains, 11 of C.jeikeium strains, 3 of C.resistens strains, 2 of C.aurimucosum strains, 2 of C.amycolatum strains and 2 of C.glucuronolyticum strains. Four isolates could not be identified into any species by 16S rRNA gene sequencing, but they were identified as C.mucifaciens (2 isolates), C.singulare (1 isolate) and C.pseudodiphtherititum (1 isolate) by MALDI-TOF MS. Conclusion:MALDI-TOF MS should be available for rapid identification of Corynebacterium spp.

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徐志红,周宏伟,孙谦,胡燕燕,陈功祥,张嵘.用MALDI-TOF MS技术快速鉴定临床分离棒状杆菌属细菌[J].临床检验杂志,2012,30(9):668-670

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  • 收稿日期:2012-02-25
  • 最后修改日期:2012-05-23
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  • 在线发布日期: 2012-10-26
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